Salinity Fluctuation on the Genetic Regulatory Mechanisms of the Crustacean, Charybdis japonica

The intertidal salinity fluctuations provide pose a significant physical challenge for the crustaceans that live here. In present study, Charybdis japonica was selected as research species to explore genetic regulatory mechanisms of responding fluctuations. We cultured C. s with three gradients (the 15‰, 25‰, and 35‰) 68.90 Gb clean transcriptome reads were obtained by RNA-seq. All then de novo assembled 41,058 unigene mean length 1,179.39 bp an N50 2,033 bp. Furthermore, total 18,100 (24.74%) unigenes successfully matched sequences from at least one database. further analyzed structure 26,853 CDSs 12,190 SSRs predicted. gene expression levels 25‰ used control, 204 5,392 DEGs found 35‰ respectively. Based on annotated information DEGs, we speculated japonicas subjected greater stress under isotonic intracellular regulation activated. neuronal activity, oxidation response, activity osmotic regulation-related genes be critical cope results will fundamental revealing fluctuation-related other crustaceans, help us predict how future changes affect survival crustaceans.